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Comparison of the rates of leading- and lagging-strand synthesis with the complete replication system as described in Materials and Methods. After incubation at 30°C, aliquots of the reaction were removed and quenched at the indicated time points, and the incorporation of [α-32P]dGMP or [α-32P]dCMP was quantitated as a measure of the rate of synthesis of either leading- or lagging-strand synthesis, respectively.
