TABLE 1.
Oligonucleotides
| Name (polarity) | Sequence (5′-3′)b | Positiona | Use |
|---|---|---|---|
| A2 (−) | TAAGCGTAGCATAGGGTAG | 11557-11575 | RT-PCR |
| A3 (−) | CCGCTGTAAATGCCATAG | 11610-11627 | RT-PCR |
| 439 (−) | CGGGATCCACCTAAAAATCCCGTCACCAC | 11923-11943 | Production of αGLC |
| 480 (−) | CGGGATCCAACACAACTATGCCG | 11493-11511 | Sequencing |
| 750 (+) | CGGGATCCTCCGCCAATTACTGTGGTT | 11684-11705 | Production of αGLC |
| JV1 (−) | TTACGACTGGTACGTTGGG | 11120-11138 | RT-PCR |
| JV2 (−) | GATACTCACCTAAAATCCCG | 11930-11949 | RT-PCR |
| 983 (−) | GAGAATTCACGGCCATACCAACAGGTTTTACTGGCGGAAC | 11315-11340 + 11482-11493 | Generation of EAV-GLΔ |
| 984 (+) | CTTGCGTGCAAGTGGTTG | 10628-10637 | Generation of EAV-GLΔ and RT-PCR |
a
Nucleotide sequence of the Utrecht variant of the EAV CVL Bucyrus strain.
b
The 5′ extensions used to introduce BamHI enzyme recognition sites are in boldface.