TABLE 1.
Coreceptors used by HIV-1 subtype CRF01_AE isolates
| Virus isolate phenotype (isolate name/yr of isolation) | Subtype (MT-2 assay/ X4a) | % Inhibi- tion by RANTES/ R5b | Coreceptor preference (X4 and/or R5) |
|---|---|---|---|
| NSI | |||
| Neutralization sensitive | |||
| CM235/1991 | E | 94 | R5 |
| NP1601/1996 | E | 100 | R5 |
| NI1149/1996 | E | 100 | R5 |
| NP1251/1998 | E | 100 | R5 |
| NP1668/1997 | E | 100 | R5 |
| NP1635/1997 | E | 100 | R5 |
| Neutralization resistant | |||
| NP1460/1997 | E | 100 | R5 |
| 2007sem/1998 | E | 100 | R5 |
| NP1564/1996 | E | 100 | R5 |
| ACT-66/1998 | E | 96 | R5 |
| BaL/controlc | B | 83 | R5 |
| SI | |||
| Neutralization sensitive | |||
| NP1636/1997 | E | 0 | X4 |
| NP1525/1997 | E | 0 | X4 |
| NP1545/1996 | E | 0 | X4 |
| NP1449/1998 | E | 0 | X4 |
| Neutralization resistant | |||
| NP1589/1996 | E | 50 | R5/X4 |
| NI1043/1996 | E | 0 | X4 |
| NP1453/1997 | E | 0 | X4 |
| NP1565/1996 | E | 0 | X4 |
| NP1604/1996 | E | 0 | X4 |
| NP1067/1996 | E | 16 | X4 |
| NP03/controlc | E | 0 | X4 |
a
The use of X4 was deduced by viral induction of syncytium formation in MT-2 cells.
b
Usage of R5 was assessed by ≥50% reduction of cell infection by RANTES.
c
The NSI subtype B virus, HIV-1 BaL, was used as a control for R-5, and the cell line-adapted SI subtype E HIV-1 NP03 isolate was used as a control for X4.