FIG. 3.

Improved infection of (primary) human cells with Ad35-based viruses. Cells were exposed for 2 h, in triplicate, to Ad5 or Ad35 vector carrying green fluorescent protein (GFP) as a marker gene, after which the medium was replaced by culture medium without virus. A549 cells, SMC, and human synoviocytes were infected at a multiplicity of infection of 500 (white bar), 2,000 (grey bar), or 5,000 (black bar) vp/cell. Mo-DC (from three different donors; data were pooled) were infected with 100, 1,000, or 2,500 vp/cell. After 48 h cells were harvested and gene transfer efficacy was determined.