FIG. 4.

Biodistribution of recombinant Ad35 vectors in mice. (A) Ad5.AdApt.Luc or Ad35ΔE3.AdApt.Luc (10¹¹ vp) was injected i.v. into BALB/c mice, and luciferase expression was monitored 72 h later using charge-coupled device camera technology (56). (B) Quality of Ad5 and Ad35 vector batches. Human A549 cells were exposed for two hours to an increasing concentration of Ad5 or Ad35 carrying the luciferase reporter gene. Cells were harvested after 48 h and lysed, and luciferase activity was determined (expressed in relative light units [RLU]).