FIG. 5.

Ad35 vectors circumvent Ad5 immunity. (A) Human serum samples were tested for NA against Ad5- or Ad35-luciferase viruses. Black dots represent individual sera at the serum dilution that inhibited >90% of the luciferase activity in the absence of serum. The bars represent geometric mean titers. (B) Serum samples from mice twice immunized with Ad5.empty viruses (10¹⁰ vp) were tested for NA against Ad5- or Ad35-luciferase viruses. (C) Spleen cells from mice twice immunized with Ad5.empty viruses (10¹⁰ vp) were incubated with NIH 3T3 cells transduced with either Ad5 or Ad35 empty vectors and IFN-γ release was quantified.