FIG. 5.

Gold immunolabeling of purified RTCs (4 h postinfection) with anti-Vpr polyclonal antibody (A), with anti-IN antibody (B and C) or nonimmune rabbit serum (D), followed by incubation with 5-nm colloidal gold-conjugated secondary antibody. Samples were stained with ethanolic UA in panel A or STA (pH 7.6) in panels B, C, and D. The arrows in panels B and C point to small clusters of gold particles. The round white dots in panels B and C are sublimation artifacts of the STA staining. The circled area in panel D contains a gold particle and is magnified in the inset. Scale bar, 100 nm.