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Purification of the HDAC3 complex. (A and C) Silver-stained SDS/polyacrylamide gel of the HDAC3 complex. (B and D) Histone deacetylase activity assayed from immunoaffinity-purified complexes. Each assay was performed in duplicate, and the values shown are the averages. w = wash, e = eluate, e + c = eluate and competitor.
