Figure 2.

In vitro association of HDAC3 with N-CoR. (A) Schematic drawing of N-CoR. The ability of each N-CoR fusion protein to bind HDAC3 is indicated (+ or −). Representative autoradiograms of (B) in vitro translated Gal4-N-CoR protein captured by GST-HDAC3 fusion protein, (C) in vitro translated HDAC3 protein captured by GST-N-CoR fusion proteins, and (D) in vitro translated HDAC4 and HDAC5 proteins captured by GST-N-CoR fusion proteins. Several independent experiments yielded consistent results. The input lanes were loaded with one-tenth the amount of ³⁵S-labeled proteins used in the binding reactions.