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Thermal inactivation of chimeric viruses. Thermal stability of chimeric virus particles was determined by incubation of 5,000 PFU (diluted in PBS supplemented with Ca2+ and Mg 2+) at 56°C for the times indicated. Aliquots were taken at the indicated time points, and virus titers were determined by plaque titration on BHK cell monolayers. The wild-type (wt) virus, Toto71, was included for comparison. Values are averages from four independent experiments.
