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Electron microscopy of in vitro-assembled CLPs. E. coli-expressed capsid protein was mixed with a 48-mer DNA oligonucleotide at a molar ratio of 1:1 or 1:2 in assembly buffer and incubated at room temperature for 15 min. The assembly reaction mixtures without further purification were negatively stained and examined by electron microscopy by the method of Mukhopadhyay et al. (27).
