TABLE 1.
Characteristics of chimeric viruses
| Virus | Residue at positiona
|
No. of amino acidsb | No. of heptad repeatsc | Plaque phenotyped | |
|---|---|---|---|---|---|
| a | d | ||||
| SINV (wild type) | V | L | 2.5 | LP | |
| SINV/WEEV(34-56) | V/I | L | 23 | 2.5 | LP |
| SINV/GCN4(255-277) | V/N | L | 23 | 3.3 | MP |
| SINV/GCN4(255-277)(L268D) | V/N | L(L268D) | 23 | 3.3 | NR |
| SINV/GCN4(250-281) | V/N | L | 33 | 4.5 | LP |
| SINV/GCN4(250-281)(L268D) | V/N | L(L268D) | 33 | 4.5 | SP |
| SINV/GCN4 tri(250-281) | I | I | 33 | 4.5 | SP |
| SINV tri(38-55) | V | V | 4 | 2.5 | VSP |
a
Residue at position a or d of the helical wheel.
b
Number of amino acids substituted for the wild-type SINV helix I and flanking region.
c
One heptad repeat defined from the a position of the helical wheel to the next a position. It is not equivalent to the number of amino acids substituted, since flanking residues were included in certain cases.
d
The plaque phenotype was determined at 48 h postinfection. Abbreviations: LP, large plaque (3.5 to 4.0 mm in diameter); MP, medium plaque (2.5 to 3.5 mm); SP, small plaque (2.0 to 2.5 mm); VSP, very small plaque (<2.0 mm); NR, not recovered.