TABLE 2.
NC assembly by chimeric viruses
| Virus | In vivo NC assemblya | In vitro CLP assemblyb | Inhibition of WT CLP assembly (in vitro) |
|---|---|---|---|
| SINV (wild type) | Yes | Yes | |
| SINV/WEEV(34-56) | Yes | NDc | ND |
| SINV/GCN4(255-277) | No | No | Yes |
| SINV/GCN4(250-281) | Yes | Yes | No |
| SINV/GCN4 tri(250-281) | Yes, with aggregation | Mainly aggregation | No |
a
NC assembly was determined by sucrose gradient sedimentation of cytoplasmic extracts from infected cells and by thin-section electron microscopy of infected cells.
b
CLP assembly was determined by agarose gel analysis and electron microscopy.
c
ND, not determined.