. 2003 Aug;77(15):8216–8226. doi: 10.1128/JVI.77.15.8216-8226.2003

TABLE 2.

Plasmids used in two-hybrid assays

Plasmid	Expressed protein	Description of plasmid construction^a
		Template	Primer
		Template	Name	Sequence (5′→3′)
pKT25PR-S^b	T25-N7-PR_D25N-C7	pKACPR_D25N	P9	gcgggatcctcatatgGGAACTGTATCCTTTAAC
pKT25B3-S^b	T25-N7-B3_D25N-C7	pKACB3_D25N	P10	cgcggtaccAGTTTCAATAGGAC
pKT25PR-L^b	T25-N22-PR_D25N-C29	pKACPR_D25N	P11	gcgggatcctcatatgGGTAGAGACAACAA
pKT25B3-L^b	T25-N22-B3_D25N-C29	pKACB3_D25N	P12	cgcggtaccTTCTTCTGTCAATGGCCATTG
pKT25PR-L/S^b	T25-N22-PR_D25N-C7	pKACPR_D25N	P11	gcgggtacctcatatgGGTAGAGACAACAA
pKT25B3-L/S^b	T25-N22-B3_D25N-C7	pKACB3_D25N	P10	cgcggtaccAGTTTCAATAGGAC
pKT25TFPR^b	T25-TF-PR_D25N-C7	pKACTFPR_D25N	P15	ggggatccgTTTTTTAGGGAAGATC
pKT25TFB3^b	T25-TF-B3_D25N-C7	pKACTFB3_D25N	P10	cgcggtaccAGTTTCAATAGGAC
pST18CPR-S^c	T18-N7-PR_D25N-C7	pKACPR_D25N	P1	gcggtcgactcatatgGGAACTGTATCCTTTAAC
pST18CB3-S^c	T18-N7-B3_D25N-C7	pKACB3_D25N	P2	cgcggatccAGTTTCAATAGGAC
pST18CPR-L^c	T18-N22-PR_D25N-C29	pKACPR_D25N	P13	gcggtcgactcatatgGGTAGAGACAACAAC
pST18CB3-L^c	T18-N22-B3_D25N-C29	pKACB3_D25N	P6	gcgcccggatccccttacccTTCTTCTGTCAATGG
pST18CPR-L/S^c	T18-N22-PR_D25N-C7	pKACPR_D25N	P13	gcggtcgactcatatgGGTAGAGACAACAAC
pST18CB3-L/S^c	T18-N22-B3_D25N-C7	pKACB3_D25N	P2	cgcggatccAGTTTCAATAGGAC
pST18CTFPR^c	T18-TF-PR_D25N-C7	pKACTFPR_D25N	P16	gggtcgacgTTTTTTAGGGAAGAT
pST18CTFB3^c	T18-TF-B3_D25N-C7	pKACTFB3_D25N	P2	cgcggatccAGTTTCAATAGGAC

The plasmids were constructed by PCR amplification of DNA sequences using the indicated templates with corresponding primers (capital letters correspond to HIV Gag-Pol sequence, and underlined sequences correspond to the restriction sites used for subcloning of PCR products). The PCR products were then digested and inserted between the corresponding sites of pKT25 (7) or pST18C. Plasmid pST18C expresses the T18 fragment of AC (aa 225 to 400) under the control of a constitutive promoter (see Materials and Methods).

Product inserted between corresponding sites of pKT25.

Product inserted between corresponding sites of pST18C.