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. 2003 Aug;77(15):8290–8298. doi: 10.1128/JVI.77.15.8290-8298.2003

FIG. 6.

FIG. 6.

The CD83 promoter is activated by LMP1 expression plasmids in transient transfections. (A) Fifty nanograms of CD83 promoter-reporter constructs were transfected into 293-T cells together with 0.5 μg of LMP1 and 10 ng of β-galactosidase expression plasmids (black bars). (B) The LMP1 expression plasmid (pSV-LMP1) or LMP1 mutant expression plasmid pSV-LMP1 (Δ194-386), pSV-LMP1 (PQT→AAA), pSV-LMP1 (Y384G), or pSV-LMP1 (PQT→AAA/Y384G) (0.5 μg) and 10 ng of β-galactosidase reporter plasmid were cotransfected with 50 ng of the CD83 promoter-reporter construct (−261) or pGL Basic, as indicated by black and white bars, respectively. Transfections were harvested the following day, and the luciferase values were normalized, with β-galactosidase as a standard. The CD83 promoter activity is given as fold induction versus mock-transfected controls. Error bars show standard deviations for at least three independent experiments.