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. 2003 Aug;77(15):8187–8195. doi: 10.1128/JVI.77.15.8187-8195.2003

FIG. 3.

FIG. 3.

Viral gene expression and production of infectious virions in HCMV-infected EVT. (A) EVT infected with HCMV at an MOI of 5. Scale bar; 5 μm. (B) Expression of HCMV genes in infected EVT. EVT were infected with HCMV at an MOI of 5, and the expression of immediate-early (IE1/IE2), early (UL112-113), and early-late (pp65) proteins was examined by indirect immunofluorescence microscopy (C) Expression of IE1/IE2, UL112-113, and pp65 in HCMV-infected EVT and HEL. EVT and HEL were infected with HCMV at an MOI of 1 or 5 and were examined by the indirect immunofluorescence method at 24, 48, and 72 hpi. (D) Production of infectious virions in EVT and HEL. The single-step growth curve of HCMV in EVT and HEL after infection of HCMV at an MOI of 5.