FIG. 1.

NF-κB(p65) inhibits activation of gammaherpesvirus lytic promoters. (A) Diagram of firefly luciferase (lucif.) reporter constructs used in transient-transfection assays. Constructs represent lytic genes from KSHV (pRpLuc and pPAN-69Luc), MHV68 (57pLuc), and EBV (BHLF1Luc). All reporters are highly inducible by viral immediate-early transactivators. Characterized binding sites for RTA (R) and Zebra (Z) are indicated. (B) 293T cells transiently transfected with the reporter constructs described in panel A. Cells were transfected with a fixed amount (+) of vectors expressing KSHV, MHV68, or EBV RTA/Zebra and increasing amounts of pFLAGp65. Luciferase assay data from three separate experiments are shown. Reporter activity is expressed as a percentage of activation, with activation by RTA or RTA and Zebra alone = 100%.