FIG. 2.

Inhibition of transactivation by NF-κB(p65) is reversible. 293T cells were transfected with the reporter constructs shown in Fig. 1A and plasmids expressing transactivator(s) RTA or RTA and Zebra. Amounts of viral transactivators were increased 4-fold (pPAN-69Luc), 5-fold (BHLF1Luc), or 10-fold (pRpLuc and 57pLuc [++]) to restore activation in the presence of p65. I-κBS32/36 is a dominant inhibitor of NF-κB.