FIG. 3.

NF-κB(p65) inhibits replication of MHV68. (A) Western blot of 293T whole-cell extracts harvested 5 days posttransfection with purified MHV68 virion DNA and pCMVp65. Membranes were probed with polyclonal serum recognizing multiple MHV68 lytic antigens (upper panel) and actin MAb (lower panel). (B) MHV68 titer in supernatant from cells described for panel A. Viral titers from three separate transfection experiments are represented. (C) Phase-contrast images of cells described in panels A and B 5 days posttransfection.