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Inhibition of MHV68 replication by NF-κB(p65) is reversible. Western blot of 293T cells transfected with 1 μg of MHV68 virion DNA and 1 μg of pCMVp65 as in Fig. 3. Blots were probed with polyclonal serum against MHV68 lytic antigens (upper panel) and MAbs recognizing p65 (center panel) and actin (lower panel). p.t., posttransfection.
