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. 2003 Aug;77(15):8532–8540. doi: 10.1128/JVI.77.15.8532-8540.2003

FIG. 6.

FIG. 6.

Both the activation domain and DNA binding domain of p65 are required for inhibition of MHV68 replication. (A) Western blot of 293T whole-cell extracts 5 days posttransfection with MHV68 virion DNA and pFLAGp65 constructs as described in the legend to Fig. 5. Blots were probed with polyclonal serum recognizing multiple MHV68 lytic antigens and a MAb recognizing actin. (B) MHV68 titers in supernatants from cells described for panel A. Average titers from three separate transfection experiments are represented. (C) Phase-contrast and fluorescence images of cells described for panel A, 2 days posttransfection with a recombinant MHV68 strain expressing GFP.