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. 2000 Jun 20;97(13):7208–7213. doi: 10.1073/pnas.97.13.7208

Figure 1.

Figure 1

(A) SDS/PAGE analysis of the purification of XDH. Lane 1, marker (Novex See Blue); lane 2, 10 μg C. purinolyticum extract; lane 3, 10 μg DEAE-1 pool; lane 4, 10 μg phenyl-Sepharose pool; lane 5, 10 μg DEAE-2 pool; lane 6, 10 μg CL6B pool; lane 7, 10 μg DEAE-3 pool; lane 8, 5 μg CM pool (purified XDH). Arrows indicate large, medium, and small subunits. (B) SDS/PAGE analysis of the purification of PH. Lane 1, marker (Novex See Blue); lane 2, 10 μg C. purinolyticum extract; lane 3, 10 μg DEAE-1 pool; lane 4, 10 μg phenyl-Sepharose NH4SO4 precipitation pool; lane 5, 10 μg CL6B pool; lane 6, 10 μg DEAE-2 pool; lane 7, 10 μg SW4000XL pool; lane 8, 10 μg DEAE-3 pool; lane 9, 5 μg SW3000XL pool (purified PH). Arrows indicate four subunits. Numbers to the left in both A and B represent molecular mass in kDa of proteins present in the standard.