Figure 4.

Inactivation of PH by KCN. Specific activity of PH was determined by using hypoxanthine as the substrate at a concentration of 100 μM and DCIP as electron acceptor at 50 μM as described in Materials and Methods. KCN was added to give 1 mM final concentration to PH in storage buffer and samples were taken at the indicated times. The value plotted represents the fraction of the initial activity. Three independent experiments were carried out and the error bars represent one SD from the average value.