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Substrate specificity of XDH
| Substrate | Substrate concentration, mM | Specific activity, μmol· min−1⋅mg−1 |
|---|---|---|
| Xanthine (control) | 0.5 | 2.6 |
| Hypoxanthine | 1.0 | 1.2 |
| 2OH-purine | 2.0 | 2.3 |
| Allopurinol | 2.0 | 1.5 |
| 1-Methylxanthine | 2.0 | 2.6 |
| 7-Methylxanthine | 2.0 | ND |
| 8-Methylxanthine | 2.0 | ND |
| Purine | 2.0 | 0.7 |
| Adenine | 2.0 | ND |
| Guanine | 2.0 | ND |
| Nicotinic acid | 2.0 | ND |
ND, activity not detectable.
