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Kinetic parameters of XDH
| Substrate | Km, μM | Vmax, μmol· min−1⋅mg−1 | Vmax/Km |
|---|---|---|---|
| Xanthine | 4.5 | 2.7 | 0.6 |
| Hypoxanthine | 500 | 1.2 | 0.0024 |
| Allopurinol | 1000 | 2.0 | 0.0020 |
The approximate Km value was estimated by using a Michaelis–Menten plot and confirmed experimentally after this determination. The Vmax was estimated from a Michaelis–Menten plot.
