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Kinetic parameters of PH
| Substrate | Km, μM | Vmax, μmol· min−1⋅mg−1 | Vmax/Km |
|---|---|---|---|
| Purine | 4.0 | 1.0 | 0.25 |
| Hypoxanthine | 5.0 | 2.0 | 0.40 |
| 2OH-purine | 50 | 1.3 | 0.026 |
| Allopurinol | 40 | 1.3 | 0.033 |
| Benzaldehyde | 200 | 0.7 | 0.004 |
The approximate Km value was estimated by using a Michaelis–Menten plot and confirmed experimentally after this determination. The Vmax was estimated from a Michaelis–Menten plot.
