FIG. 2.
Expression of gD, gE, gB, and gI by recombinant HSV-1. Human keratinocyte HaCaT cells were infected with wild-type HSV-1, F-US6kan/gE-GFP, F-US6kan, F-gE-GFP, vRR1097, vRR1097/gEβ, F-gDβ, F-gDβ/gE-GFP, or F-gEβ or were left uninfected. The cells were labeled with [35S]methionine-cysteine, and gD, gE, gB, or gI was immunoprecipitated from cell extracts by using MAb DL6, 3114, 15βB2, or 3104, respectively. Antigen-antibody complexes were collected by using protein A-Sepharose, and eluted proteins were subjected to electrophoresis on 12% polyacrylamide gels. Panels A, B, and C include all the mutants except vRR1097/gEβR; gD was precipitated in panel A, gE was precipitated in panel B, and gB was precipitated in panel C. In panel D, vRR1097/gEβR was characterized for gD, gE, and gI. The positions of mature and immature forms of gE, gD, and gB as well as marker proteins of 200, 97.4, 68, 43, 29, and 18.4 kDa are indicated.