TABLE 2.
Distribution of virus particles produced by wild-type and mutant HSV-1 in HEC-1A cellsa
| Virus | No. (%) ofb:
|
|||
|---|---|---|---|---|
| Nucleocapsids in:
|
Enveloped virions:
|
|||
| Nucleus | Cytoplasm | In cytoplasm | On cell surface | |
| Wild type | 393 (43.9) | 30 (3.3) | 36 (4.0) | 435 (48.6) |
| F-gEβ | 335 (44.5) | 75 (9.9) | 97 (12.8) | 246 (32.6) |
| vRR1097 | 414 (52.5) | 36 (4.6) | 45 (5.7) | 293 (37.1) |
| vRR1097/gEβ | 268 (25.2) | 695 (65.6) | 12 (1.2) | 85 (8) |
| F-gDβ/gE-GFP | 374 (22.4) | 1,227 (73.4) | 32 (1.9) | 38 (2.3) |
| VRR1097/gEβ-R | 389 (56.3) | 44 (6.3) | 38 (5.4) | 220 (31.8) |
a
Human HEC-1A cells were infected with wild-type or mutant HSV-1 for 16 h. The cells were fixed, sectioned, and then examined by electron microscopy.
b
The numbers of unenveloped nucleocapsids in the nucleus and in the cytoplasm and enveloped virions in the cytoplasm and on the cell surface were counted in approximately 10 to 15 randomly chosen cells. The numbers in parentheses are percentages of the total numbers of particles.