TABLE 1.
Affinities of different CXCL8 analogs for vCKBP-3a
| Analog | Kd (pM) |
|---|---|
| WT CXCL8 (residues 1-72) | 23.54 ± 8.61 |
| Truncations of CXCL8 (residues contained) | |
| 4-72 | 33.38 ± 9.39 |
| 7-72 | 37.85 ± 1.81 |
| 1-69 | 25.42 ± 4.41 |
| 1-66 | 34.5 ± 8.90 |
| 1-58 | 520.33 ± 8.09 |
| Substitutions within CXCL8 | |
| I10A | 288.25 ± 67.86 |
| L49A | 118.06 ± 18.22 |
| Q8P | 71.85 ± 14.41 |
| K15A | 45.53 ± 9.25 |
| A35P | 33.68 ± 9.30 |
| H18L | 34.69 ± 1.14 |
| Hybrid analogs | |
| WT CXCL10 | 232.48 ± 37.76 |
| CXCL10-CXCL8H1 | 241.03 ± 24.2 |
| CXCL10-CXCL8H7 | 33.49 ± 5.36 |
| WT CXCL4 | >b |
| CXCL4-CXCL8H1 | 69.05 ± 19.73 |
| WT CXCL12 | > |
| CXCL12-CXCL8H1 | > |
a
Analogs that have substitutions within CXCL8 are based on the truncated CXCL8 containing residues 4 to 72.
b
>, undetectable binding.