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Alteration by MAbs of VCP's complement inhibitory activity. Serial dilutions of rVCP were incubated with human serum in the presence or absence of MAb and then added to the wells containing immune complexes. The amount of C3b deposited was detected by using anti-C3 antibody. Since the reactivity of all of the MAbs within each of the two groups of MAbs was similar, representative data are shown for MAbs 3B1 (nonblocking) and 3D1 (blocking). O.D., optical density.
