FIG. 5.

(A) Nucleotide (upper bars) and amino acid (lower bars) substitutions into the genomes of cVDPV isolates Qalyubiya/88-1 (above) and Beni Suef/93 (below). The reference sequence is that of Sabin 2. The substitution maps are aligned with a schematic of the poliovirus genome; the single open reading frame is indicated by a rectangle, flanked by the 5′ and 3′ UTRs. Asterisks indicate attenuating mutations replaced by recombination (5′ UTR) or nucleotide substitution (VP1). (B) Neighbor-joining trees summarizing sequence relationships in the 5′ UTR and in each interval encoding a major cleavage product from the poliovirus polyprotein; all trees are drawn to the same scale. Isolate sequence abbreviations: S2, Sabin 2; QL, Qalyubia/88-1; BS, Beni Suef/93.