TABLE 2.
PCR and sequencing primers
| Primera | Target sequence (nucleotides) | Primer sequenceb |
|---|---|---|
| 1S | 1-19 | TTAAAACAGCTCTGGGGTT |
| 446Sc | 446-470 | TCCGGCCCCTGAATGCGGCTAATCC |
| 559Ac | 533-559 | ACACGGACACCCAAAGTAGTCGGTTCC |
| 636A | 613-636 | GGATGGCCAATCCAAYTCGCTTTA |
| 780A | 761-780 | TCCAACTTTTTGAGATGAAA |
| 823S | 823-846 | AATTACACTACAATCAATTACTAT |
| 1018A | 995-1018 | TTGAATTGCCCAGAGTTAGCTGCA |
| 1135S | 1135-1156 | TGCAGGTTCTACACATTAGATA |
| 1493A | 1472-1493 | AAGAGGTAATCAACTGGGCAGA |
| 1681S | 1681-1700 | TCCACTGAGATACCCATTAC |
| 1773A | 1752-1773 | TAATCCTTGGGTTCTTGGCACA |
| 1951S | 1951-1974 | AAGAACACAATGGACATGTATAGG |
| 1973A | 1954-1973 | CTATACATGTCCATTGTGTT |
| 2031A | 2011-2031 | GGACAACGAGAGACACAAGAT |
| 2401Sd | 2401-2423 | GGTTTTGTGTCAGCGTGTAATGA |
| 2507Se | 2507-2526 | CCGTTGAAGGGATTACTAAA |
| 2577Ae | 2562-2577 | CGGCTTTGTGTCAGGC |
| 2858Sd,f | 2858-2877 | CITAITCIMGITTYGAYATG |
| 2899S | 2899-2922 | TCAAACTATACTGATGCAAACAAC |
| 2936Ad,f | 2917-2936 | TTIAIIGCRTGICCRTTRTT |
| 3008A | 2986-3008 | TGCCACGTATAGTCATTCCATTT |
| 3356S | 3356-3375 | CACTACCAGAAAAGGGATTA |
| 3418A | 3397-3418 | CTGTATACACAGCTTTGTTTTG |
| 3503Ad | 3484-3503 | AAGAGGTCTCTRTTCCACAT |
| 3838S | 3838-3858 | TCCAACTACATTGAGTCCCTT |
| 4129S | 4129-4149 | AGTTGGTTGAAGAAATTCACA |
| 4411S | 4411-4431 | GAAGCAAAAAGGATTCAGAAG |
| 4496A | 4475-4496 | AAACATACTGGCTCAATACGGT |
| 4957S | 4957-4977 | AAGAGATGTTGCCCTTTAGTG |
| 5036A | 5017-5036 | TGATCAATGCTGTATCTAAC |
| 5456S | 5456-5479 | CAGTGGCCATGGCTAAGAGAAACA |
| 5555A | 5533-5555 | TGGGTTGGTAGAATAGCCACGTT |
| 5953S | 5953-5974 | GCCCTGAAGCGATCATACTTCA |
| 6104A | 6084-6104 | GGTTCCTTAACTCCTTCAAAC |
| 6539S | 6539-6560 | TGGCCATGAGAATGGCATTTGG |
| 6656A | 6636-6656 | AGCACTGGGATTTTGCTCCAA |
| 7078S | 7078-7101 | ACAGTCACATGGGAGAATGTAACA |
| 7195A | 7175-7195 | TCCATCTAATTGATTCATGAA |
| 7241S | 7241-7263 | TATTGGCCTGGCACAATGGTGAA |
a
A, antisense polarity; S, sense polarity. Numbering of nucleotide positions follows that for the Sabin 2 sequence (49).
b
Bases in inosine-containing and degenerate primers: I, inosine; R, A + G; Y, C + T.
c
Primers previously described as EV/PCR-2 (446S) and EV/PCR-1 (559A) (76).
d
Primers used for VP1 sequencing.
e
Primers previously described as Sabin 2/PCR-2 (2507S) and Sabin 2/PCR-1 (2577A) (75).
f
Primers previously described as panPV/PCR-2 (2858S) and panPV/PCR-1 (2936A) (27).