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Replication kinetics of wt HIV-1 and DIS stem-loop mutants passaged through PBMCs in Sup T1 cells. SupT1 cells were infected with either NL4.3 wt (▪) or mutant viruses (NLGCGCGC [Δ] and NLACGCGT [□]) that had been passaged through PBMCs. Supernatants were collected 3, 7, 10, and 14 days after infection, and RT activity in each sample was measured. Results represent means and standard deviations of triplicate samples and are representative of eight sets of experiments.
