TABLE 2.
RT and p24CA properties of NC mutant and wild-type HIV-1
| Mutanta | RT (cpm/ml)b | Mean p24CA (ng/ml)c level ± SD |
|---|---|---|
| (−)-Controld | 0 | 0 |
| CNBP-1 | (4.0 ± 1.0) × 106 | 220 ± 50 |
| CNBP-2 | (2.1 ± 0.1) × 106 | 100 ± 10 |
| CNBP-3 | (2.8 ± 0.9) × 106 | 130 ± 10 |
| CNBP-4 | (1.9 ± 0.2) × 106 | 140 ± 10 |
| CNBP-5 | (1.9 ± 0.4) × 106 | 100 ± 30 |
| CNBP-6 | (1.8 ± 0.1) × 106 | 40 ± 20 |
| CNBP-7 | (2.3 ± 0.3) × 106 | 40 ± 20 |
| Wild-type HIV-1 | (2.3 ± 0.2) × 106 | 50 ± 30 |
a
Mutants are described in Table 1.
b
Counts per minute of [3H]TMP incorporated per milliliter of culture fluid. Values represent the average of two replicates from a single harvest.
c
Determined by p24CA antigen ELISA from the AIDS Vaccine Program, Biological Products Laboratory. Values represent the average of five replicates from a single harvest.
d
(−)-Control is from cultured cells transfected with sheared salmon sperm DNA.