TABLE 3.
Reverse transcription intermediate levels from mutant and wild-type virus infections
| Virus | Mean level ± SDa of:
|
|||
|---|---|---|---|---|
| SS | FST | Gag | SST | |
| (−)-Controlb | 0.0 | 0.0 | 0.0 | 0.0 |
| CNBP-3 | 1.2 ± 0.1 | 1.3 ± 0.3 | 1.4 ± 0.3 | 1.1 ± 0.2 |
| CNBP-5 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.03 ± 0.01 | 0.04 ± 0.01 |
| Wild type | 1.0 | 1.0 | 1.0 | 1.0 |
a
Values are reported for each reverse transcription intermediate relative to values obtained from the wild-type virus. The average of two independent transfection-infection experiments is presented. SS is the minus-strand strong-stop DNA, the R/U5 target sequence (7). FST is first-strand transfer vDNA, the U3/U5 target sequence. Gag is the gag target sequence. SST is second-strand transfer vDNA, the R/5′-untranslated region target sequence.
b
Mock infection was done with clarified sheared salmon sperm DNA transfection supernatants.