FIG. 2.

HCMV enters into the cytoplasm in the absence of the MT network. Effects of nocodazole removal on expression levels of IE1 were examined with HEL cells infected with HCMV at an MOI of 3 PFU/cell. (A) Schematic representation of sequential treatments with nocodazole, treatment with proteinase K (PK), and inoculation. C1 and C2, controls 1 and 2. (B) Lysates were taken at the times indicated in panel A and subjected to Western blotting analysis to compare IE1 antigens. Relative signal intensities of IE1 normalized by signals of beta-actin are shown. Values represent means ± standard deviations (n = 3 or 4).