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pUL83 release in nocodazole-treated HCMV-infected cells. HEL cells were mock infected (a and b) or infected with HCMV at an MOI of 5 PFU/cell in the absence (c and d) or presence (e and f) of 10 μM nocodazole. At 2 hpi, the cells were fixed and stained for pUL83 (a, c, and e). Nuclei were visualized with TOPRO3 (b, d, and f). Bars, 50 μm.
