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TABLE 3.

Range of VBSs tested to determine optimum matching tolerance for E. faecium isolates

Parameter Result at VBS of:
0.125 0.500 0.750 0.925a 1.250
Match similarity percentage defining dendrogram group 7 44 66 74 73
Within dendrogram group
    Total no. of isolatesb 59 55 37 31 43
    No. of VSG isolatesc 26 26 26 26 26
    No. of dissimilar isolates (non-VSG) 33 29 11 5 17
% Sensitivityc 100 100 100 100 100
% Specificityd 6 17 31 86 49
a

Optimum VBS setting for E. faecium PFGE library.

b

Excludes reference E. faecium isolate.

c

By definition, the dendrogram group included all VSG isolates.

d

Specificity = [true negatives/(false positives + true negatives) × 100], where true negatives are non-VSG isolates located outside of the dendrogram group.