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. 2006 Nov 14;400(Pt 2):245–253. doi: 10.1042/BJ20060749

Figure 1. SDS/PAGE of recombinant wild-type and mutant AdoHcyases.

Figure 1

(a) Crude extracts from overnight (O.N.) cultures of non-induced and IPTG-induced E. coli harbouring the wild-type expression plasmid, and Ni-NTA affinity-purified wild-type AdoHcyase, (b) Ni-NTA affinity-purified thrombin-cleaved or uncleaved recombinant AdoHcyases (1 μg). M, molecular-mass standards (Roti-Mark Standard; Carl Roth, Karlsruhe, Germany), from top to bottom: β-galactosidase (119 kDa), serum albunium (66 kDa), ovalbumin (43 kDa) and carbonic anhydrase (29 kDa). +T, thrombin-cleaved protein.