Table 1. Oligonucleotides used for PCR-based cloning and site-directed mutagenesis.

The codons changed are underlined, and the nucleotides changed are shown in boldface. The cDNA sizes of amplification products are for wild-type 1337 bp, for p.Y143C 949 bp and for p.W112stop 367 bp.

	Oligonucleotide (5′–3′)
Construct	Forward	Reverse	Codon change
Wild-type	CGCCGGTACCTCTGACAAACTGCCCT	GCATCTAGAGGGTGAAACGCAGA	−
p.Y143C	CGCCGGTACCTCTGACAAACTGCCCT	ATGTTCACCTTCTCCACGGC	[8]
p.E115L	−	AGGGTCTGCAGAATGCACCACAGGT	GAG→CTG
p.W112stop	CGCCGGTACCTCTGACAAACTGCCCT	GCTGGATCCATCACAGGTACT	TGG→TGA