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T. maritima (○) and E. coli (△) DHDPS enzymes were assayed for activity in the presence of varying concentrations of (S)-lysine and saturating concentrations of pyruvate and (S)-ASA using the coupled assay. The apparent rate (vapp) was then compared with the rate of non-incubated enzyme (vcon). Each data point was measured in duplicate or triplicate and error bars show the standard deviation.
