Figure 8. UDPGA uptake in Gunn rat liver microsomes.

Effects of UGT proteins on UDPGA uptake were examined with UGT1-deficient rat liver microsomes prepared from Gunn rats. (A) UGT protein in microsomes detected by immunoblotting. (B) Uptake of [¹⁴C]UDPGA in wild-type and UGT1-deficient rat liver microsomes. Gunn rat (j/j) homozygote (squares); wild-type (+/+) normal (circles). Solid lines and dotted lines show the time course of uptake after 90 min of preload with UDPGlcNAc and with water respectively. The uptake of water-preloaded wild-type rat liver microsomes was the same as Figure 1 and Gunn rat (j/j) liver microsomes (results not shown; n=2).