Figure 2. High levels of endogenous p53 protein induced by DNA damaging agents are associated with decreased HNF4α1/2 protein and mRNA levels.
(A and B) IB (immunoblot) as in Figure 1 (C) of HepG2 cells treated with doxorubicin (0.5 μg/ml) for the times indicated. Protein levels were analysed using antibodies against HNF4α (α-445), HNF6α (H-100), HNF1α, Sp1, p53 (DO-1) or β-actin. (C) IB analysis as in (A) of whole cell extracts from HepG2 cells harvested after exposure to UV (75 J/m2 or 150J/m2) at the times indicated.