Figure 6. Co-transfection of p53 and HNF6α suggests that p53 inhibits HNF6α transcriptional activation of the HNF4α P1 promoter.

HepG2 cells were transfected with pGL3.hHNF4α promoter (2 μg), CMV.β-gal (2 μg), pcDNA3.p53wt (1 μg) and pcDNA3.HNF6α (1 μg) as indicated. Cells were harvested at 30 h after transfection. Results are means±S.D. of one representative experiment performed in triplicate. The bracket with asterisk indicates the amount of basal repression by p53 (difference between lane 1 and lane 2). Results indicate that p53 repressed the transcriptional activity of the exogenously expressed HNF6α (see text for details).