Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Lane 1, soluble fraction of BL21 (DE3)pLysS [pET15b-TcGLO1], uninduced cells; lane 2, soluble fraction of BL21 (DE3)pLysS [pET15b-TcGLO1], induced cells; lane 3, pooled fractions from nickel affinity chromatography; lane 4, pooled fractions from anion exchange chromatography (Resource-Q); lane 5, pooled fractions from anion exchange chromatography (Resource-Q) after removal of the His6-tag with thrombin protease.
