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The apo form of TcGLO1 was incubated with a 10-fold molar excess of bivalent metal ions for 10 min at room temperature. Assays contained 25 μM trypanothione hemithioacetal and 0.1 mM free T[SH]2. L. major GLO1 reconstituted activity (black bars; [11]) is shown next to the TcGLO1 data (white bars). All assays were performed in triplicate, with the mean activity relative to the nickel-activated enzymes displayed.
