Figure 5. S-4-bromobenzylglutathionylspermidine inhibition of TcGLO1.

TcGLO1 inhibition constants were determined at three fixed concentrations of the inhibitor [(S-4-bromobenzylglutathionylspermidine] and over a range of substrate concentrations (25-125 μM trypanothione hemithioacetal). Data sets for each inhibitor concentration were fitted by non-linear regression and are presented as Lineweaver–Burk transformations. Trypanothione hemithioacetal was varied in the presence of 0 (○), 3.15 (■), 6.3 (□) and 12.6 μM (●) (S)-4-bromobenzylglutathionylspermidine.