Table 1. Kinetic properties of T. cruzi GLO1.
Kinetic constants (Km and kcat) were determined as described previously [11]. Results are means±S.D. for triplicate measurements. ND, not determined.
| Hemithioacetal substrate | Km, μM | kcat, s−1 | kcat/Km×106·M−1·s−1 | kcat/Km(relative) |
|---|---|---|---|---|
| Glutathionylspermidine | ||||
| Methylglyoxal | 8.0±0.4 | 161±12 | 20 | 100 |
| Phenylglyoxal | 14±2.1 | 105±28 | 7.5 | 37.5 |
| Trypanothione | ||||
| Methylglyoxal | 109±10 | 363±33 | 3.3 | 16.5 |
| Phenylglyoxal | 207±23 | 265±31 | 1.5 | 7.5 |
| Glutathione | ||||
| Methylglyoxal | >1800 | ND | 0.0014 | 0.007 |
| Phenylglyoxal | >1800 | ND | 0.0005 | 0.0025 |