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Full-length ovine recombinant ARR (■), ARQ (◆), VRQ (●) or bovine (▲) recombinant PrP was coated directly on to ELISA plates as described in the Materials and methods section. Recombinant PrP was detected by 1.0 μg/ml purified monoclonal antibody (a) FH11, (b) SAF32, (c) A516 and (d) V24. Results shown are means±S.D. for triplicate wells.
