Table 1. Quantification of PrPC in plasma from sheep and lambs.
Quantification of ovine plasma PrPC levels by capture–detector ELISA. Plasma PrPC levels in homozygous ARR, ARQ or VRQ adult sheep (a) or young lambs (b) were determined as described in the Materials and methods section. Capture monoclonal antibodies were FH11 or A516 and detector monoclonal antibodies were biotinylated V24 or 245. The results shown are means±S.D. for five animals analysed for each genotype and age group.
| (a) | |||
|---|---|---|---|
| Plasma PrPC (ng/ml) | |||
| Antibody pair | ARR/ARR | ARQ/ARQ | VRQ/VRQ |
| FH11/V24 | 3.0±4.0 | 6.6±3.4 | 31.8±6.7* |
| FH11/245 | 3.6±0.8 | 6.4±2.2 | 5.8±3.0† |
| A516/V24 | 13.8±2.6 | 19.8±13.1 | 61.2±11.6‡ |
| A516/245 | 5.4±0.6 | 16.8±10.0 | 29.6±11.6†§ |
| (b) | |||
| Plasma PrPC (ng/ml) | |||
| Antibody pair | ARR/ARR | ARQ/ARQ | VRQ/VRQ |
| FH11/V24 | 17.2±3.0 | 11.4±2.3 | 39.4±1.4* |
| FH11/245 | 0.1±0.2† | 0.2±0.4† | 5.4±1.2† |
| A516/V24 | 38.4±10.7 | 22.2±10.1 | 63.0±31.4∥ |
| A516/245 | <0.1† | 0.8±1.3† | 9.8±1.3† |
*P≤0.003 (comparison with ARR and ARQ).
†P≤0.008 (comparison with monoclonal antibody V24 as detector).
‡P<0.001 (comparison with ARR and ARQ).
§P=0.005 (comparison with ARR).
∥P=0.019 (comparison with ARQ).